Abstract: Feeding ecology is important for understanding ecosystem dynamics, includes what, where, and when a species eats, and can vary both inter- and intraspecifically.  While much work has gone into understanding intraspecific variation in feeding ecology on a large geographic scale, little is known about whether this type of variation occurs on the scale of microhabitats.  This study explored intraspecific variation in feeding ecology across microhabitats and interspecific variation in feeding ecology within a microhabitat using Thamnophis sirtalis, the common garter snake, and Nerodia sipedon, the northern water snake, as a model system.  By studying diet and response to prey species of these T. sirtalis and N. sipedon, we asked: Do T. sirtalis utilizing different microhabitats, aquatic and terrestrial, have different feeding ecologies?  Do T. sirtalis utilizing the aquatic microhabitat have feeding ecology more similar to N. sipedon also utilizing the aquatic microhabitat?  Diet data was collected in a field survey of gut contents and prey response was measured using a behavioral assay.  Intraspecific differences across microhabitats were observed in the diet of T. sirtalis, but not in prey response.  Interspecific similarities within a microhabitat were observed in prey response, but not in diet.  This may be due to physiological differences between T. sirtalis and N. sipedon, so that although they respond to prey similarly, they are not successful at catching and eating the same prey types.  This study demonstrates that there can be intraspecific differences in feeding ecology across microhabitats, as well as interspecific similarities in feeding ecology within a microhabitat.